The Rye Platform (provided by the Technische Universität München [TUM])

A rye TILLING platform will facilitate the identification and characterization of advantageous functional alleles of important agronomic traits using both forward and reverse genetic approaches. Four different winter rye inbred lines were chosen for mutagenesis with different EMS concentrations and treatments.
In parallel an EcoTILLING approach is pursued. In this context, a highly diverse set of lines originating from Middle and Eastern European populations is screened for natural variation in candidate genes.

A second GABI rye project aims at the large scale development of rye EST sequence data (GABI RYE-EXPRESS), which - substantiated by the barley EST collections (GABI PLANT, GABI-SEED) available at IPK - will provide the profound basis for species-specific primer design for performing TILLING screens in candidate genes. Candidate genes involved in frost tolerance will be of particular interest, since allelic variation and both, phenotypic and functional analysis of those genes serves as a validation of the results obtained by a third project, GABI RYE-FROST which aims at the functional analysis of frost tolerance in rye.